The use of conventional pesticides in the water sources, however, introduces many risks to people andor the environment. Larvicidal activity of plant extracts on aedes aegypti l article pdf available in asian pacific journal of tropical biomedicine 23. Laboratory study on larvicidal activity of different plant extracts against aedes aegypti created date. Laboratory experiments were conducted to evaluate the larvicidal activity of ethanol extracts from leaves of three plants. However, a literature survey has shown that there is no report on chemical composition and larvicidal activity of the essential oil of i. Issn 23207078 larvicidal activity of lantana camara aculeata. Larvicidal and antibacterial activities of different. Department of plant protection, faculty of agriculture, omdurman islamic university. This needs a further deep investigation to identify the effective constituents for controlling mosquitoes. The results obtained show that this plant material exhibited significant activity and could be considered as potent natural larvicidal, pupicidal and repellent agent against c.
Neembased biopesticides and neem extracts have a wide range of effects against insect pests including repellence, feeding, toxicity, sterility and growth regulator activity and are relatively safe towards non target biota with only minimal risk of direct adverse effects on aquatic biota from contamination of water bodies 21, 22. The activity of crude plant extracts is often attributed to the complex mixture of active compounds. Pdf larvicidal activity of some plant extracts to larvae of. However, other studies showed that water extracts and oils possessed high larval activity. Larvicidal activities of three medicinal plant extracts were studied in the range of 4. Studies on some plant extracts for their antimicrobial.
The larvicidal properties of 16 extracts of four moroccan medicinal plants. Mosquitoes larvicidal activity of leaf extract of neem azadirachta indica amaragathavalli. Larvicidal activity of medicinal plant extracts against aedes. Ovicidal, larvicidal and antiovipositional activities of. Zhu 2008 stated that activities have been focused on plant extracts or phytochemicals as sources of agents to control mosquito. Larvicidal activity of some medicinal plant extracts. Chemical composition and larvicidal activities of azolla pinnata. Issn 23207078 larvicidal activity of lantana camara. Aqueous extracts resulting from the different parts of the plants did not possess any larvicidal activity. The results of antimicrobial activity showed that water and ethanol crude extracts were active on all the strains of pathogens tested at different concentrations, with ethanol extracts exerting more activity. Chemical composition and larvicidal activity of plant.
Larvicidal activity of some medicinal plant extracts against malaria vector anopheles stephensi. This suggested that water is a poor solvent for resolving the larvicidal components. Larvicidal efficacy of medicinal plant extracts against the. Department of biotechnology, federal university of technology, owerri, imo state, nigeria. The bioefficacy of aloe vera leaf extract and bacterial insecticide, bacillus sphaericus larvicidal activity was assessed against the first to fourth instars larvae of aedes aegypti, under the laboratory conditions. You will be redirected to the full text document in the repository in a few seconds, if not click here. Larvicidal activity of methanolic extract of the leaves of. Among the extracts tested, nine exhibited high larvicidal activity with lc 50 24 h ranging from 28 to 325 ppm. Biological activity of indigenous plant extracts as mosquito. Pdf larvicidal activity of selected plant extracts and their. Culicidae youngcheol yang, i1kwon park, eunhee kim 2, hoiseon lee 3 and youngjoon ahn 2 department of advanced organic materials engineering, chonbuk national university, chonju 561756. Antimicrobial activity of crude extracts of three medicinal. The mortality data were subjected to probit analysis to determine the lethal concentrations lc 50 and lc 90 to kill 50 and 90 per cent of the treated larvae of the respective.
In the north west of morocco, one preliminary evaluation study of the larvicidal activity of the n. Some of the plants are used traditionally as sources of insecticidal materials. This paper reports the larvicidal activity of seventeen tanzanian plant species against the malaria vector, anopheles gambiae s. Larvicidal efficacy of plant extracts against the malarial. Pdf plant extracts as potential mosquito larvicides.
Assessment of larvicidal activity of hyptis suaveolens and. In the preliminary screening, potential larvicidal activity of plants, different solvents crude extracts of three plants was noted table ii, fig. Larvicidal and igr activity of extract of tanzanian plants. Laboratory study on larvicidal activity of different plant. Evaluation of some moroccan medicinal plant extracts for.
Antibacterial activity of plant extracts 247 brazilian journal of microbiology 2000 31. Bekele j, hassanali a 2001 blend effects in the toxicity of the. The plant extract exhibited larvicidal activities on different instars i, ii, iii and iv. Larvicidal activity of extracts from three plumbago spp. Mosquito is a vector of different diseases like malaria, filariasis and dengue fever. The attached supplementary, s1 and s2 tables pdf file contains the nist 08 library. Madhura mukadam department of zoology, gogate jogalekar college, ratnagiri, maharastra, india abstractmosquitoes are the major vector for the transmission of malaria, dengue fever, yellow fever, filariasis, schistosomiasis and japanese encephalitis. In the present study, the plant extracts exhibited the presence of larvicidal activity table 1 which was a dose dependent effect. Extracts of annona species are potential antimosquito. Issn larvicidal activity of monstera adansonii plant.
Bioassay for the larvicidal activity for their larvicidal activity against different species of was carried out using who procedure with slight mosquitoes 12, 1921. The larvicidal activity of selected parts of carica papaya extracts against aedesaegypti are given in table 2. Table i asteraceae plant species collected for testing against 4th instar larvae of aedes ochlerotatus fluviatilis lutz, 1904 entry plants common name habitat a bhcbb 1. For larvicidal assay, extracts with 50 lcgreater than 750. Larvicidal activity of some plant extracts against mosquitoes dr. Ingestion toxicity of plant extracts to gbm larvae the ingestion toxicity of plant extracts to p. One hundred milligrams of each plant extract in 100 ll of hplc grade acetone was incorporated per g of freshly prepared meridic diet, to give 10% ww. Materials and methods the present study has been carried out to assess the larvicidal activity of aqueous extracts of. Natural pesticides, especially those derived from plants, are more promising in this aspect. Larvicidal activity of some plant extracts to larvae of the mosquito. The larvicidal activity of the organic extracts was found to be speciesdependent. The larvicidal activity of 80% methanol extracts of the first two plant species against the fourth instars. Evaluation of larvicidal activity of medicinal plant.
Plant extracts as potential mosquito larvicides pdf. The larvicidal activity of the different selected plant extracts were found to be dose depended. The larvicidal activity of azolla pinnata plant extracts evidently affected the early. Evaluation of larvicidal activity of medicinal plant extracts against. Debella a 2002 manual for phytochemical screening of medicinal plants. Larvicidal and repellent potential of moringa oleifera against. Culex quinquefasciatus, gaultheria oil, larvicidal, pupicidal, repellent activity. Pdf searching for potent plant larvicides, crude aqueous extract of the latex of uschar. The larvicidal activity of azolla pinnata plant extracts evidently affected. Comparative evaluation of larvicidal potentials of three plant extracts on aedes aegypti. Laboratory of chemical ecology, department of fundamental chemistry, federal university of pernambuco ufpe. The present investigations suggest the possible use of this blend of botanical extracts as an ideal ecofriendly, larvicide against ae. Plants are well known to contain a complex of chemicals with bioactive potential like deterrents or attractants. It has been increasing development of resistance to synthetic chemicals, so plant extracts may be the alternate source of mosquito control agent.
Therefore, in the present study the larvicidal activity of crude petroleum ether, chloroform and acetone. The attached supplementary, s1 and s2 tables pdf file contains the nist 08. Larvicidal activity of nerium oleander against larvae west. Bioassay procedure the larvicidal activity of the extracts was evaluated as per the method recommended by wh07. Larvicidal activity of neem oil azadirachta indica. All the selected eo showed potential larvicidal activity with lc50 for 24 h lies between 0. Pdf larvicidal activity of plant extracts on aedes aegypti l. Larvicidal activity of the extracts from different parts. Larvicidal and adulticidal effects of extracts from some indigenous.
Evaluation of mosquito larvicidal effect of carica coden. Larvicidal activity of selected plant extracts against the. Mosquito larvicidal activity of hyptis suaveolens filarial. Modern agronomy, plant breeding, agrochemicals such as pesticides and fertilizers, and technological improvements such as genetic modification in food, have sharply increased yields from cultivation. Larvicidal activity of some plant extracts to larvae of the mosquito culex. Jul 22, 2012 the bioefficacy of aloe vera leaf extract and bacterial insecticide, bacillus sphaericus larvicidal activity was assessed against the first to fourth instars larvae of aedes aegypti, under the laboratory conditions. Larvicidal activity of essential oil derived from illicium. The samples of roots, leaves and fruits were washed 23 times with distilled water, chopped and shade dried separately at a temperature between 3040 oc for 1015 days. Larvicidal activity of plant and algae extracts, essential. Larvicidal activity of some medicinal plant extracts against. Larvicidal activity of essential oils from brazilian. While some plant extracts were comparatively more effective against all the three pathogens xanthomonas campestris, agrobacterium rhizogenes and aspergillus fumigatus. Larvicidal activity of plant extracts against aedes aegypti 1217 caitied out using acetone 61 and petroleum ether 6080c, 61.
It is evident that the results on mortality of aedesaegypti show extract dependents activity. The ethanol, hexane and chloroform extracts of stem and leaf of solanum nigrum for assayed against larvicidal activity was carried out at different concentrations ranging from 500, and 1500 ppm in distilled water. The measured amount of extracts was dissolved in 1ml of the solvent which was used for preparing the extracts. Insecticidal activity of plant essential oils has been welldescribed by isman 5. The stock solutions of the plant extracts were volumetrically diluted to 250 ml with filtered tap water to obtai n the test solutions of 10, 20, 40, 60 and 80 mgl. Azadiractin, the active ingredient of neem has long been recognised for its mosquito larvicidal capability.
From the stock solution, concentrations activity are methanolic stem extracts of satureja hortensis. Larvicidal activity of medicinal plant extracts against. Plasmodium spp, the vector mosquito has to be controlled. Larvicidal against mosquito vectors and brine shrimp. Pdf larvicidal activity of some plant extracts to larvae. All these plant extracts were made free from solvent by steam distillation, concentrated by evaporation and were used for the larvicidal testing by standard procedure5. Evaluation of larvicidal properties of leaves and root. The plant neem azadirachta indica was selected for study. Preliminary evaluation of mosquito larvicidal efficacy of. Table 1 shows the percentage mortality of the 4th instar larvae of mosquito exposed for 24hrs to various concentrations of acetone extracts of the two plants.
Larvicidal activity of moringa oleifera flower extracts. Larvicidal activity of plant extracts used alone and in. Santos 2, 1department of biology, college of arts and sciences cebu normal university, osmena boulevard, cebu city, philippines. In order to develop an environmentfriendly botanical mosquito larvicide alternative to the chemical larvicides, extracts were made from the leaves of hyptis suaveolens, lantana camara, nerium oleander, and tecoma stans with three organic solvents. Plant extracts are safer for nontarget organisms including man. Conclusion from these results it was concluded that neem and pine oil were found to have larvicidal activity under lab conditions with best efficacy in terms of lc 50, lc. Larvicidal potential of three plant extracts on the. Balanitaceae, calotropis procera asclepiadaceae and eucalyptus globulus. Combinations of plant essential oil based terpene compounds as. The present paper discusses the larvicidal activity of neembased biopesticide for the control of mosquitoes. Pdf larvicidal activity of some plant extracts to larvae of the. Larvicidal activity of selected plant extracts against the dengue vector aedes aegypti mosquito pedro m. Pdf larvicidal activity of medicinal plant extracts. Jul 15, 2015 larvicidal activities of 100 indian coastal plant extracts were examined against aedes aegypti among which til oil showed 34% to 100% mortality at different concentrations.
Effect of monstera adansonii on 4th instar larvae of culex quinquefasciatus. Biological activity of indigenous plant extracts as. The toxicity of the extracts decreased with increase in larval stage. The crude extracts from the leaves, stem and root barks of the investigated plants were. Larvicidal and antibacterial activities of different solvent. The extracts of these plant have a few important chemical constituents like carbohydrates, glycoside, and tannins. Larvicidal activities of 100 indian coastal plant extracts were examined against aedes aegypti among which til oil showed 34% to 100% mortality at different concentrations. Mosquitoes in the larval stage are attractive targets for pesticides because mosquitoes breed in water, and thus, it is easy to deal with them in this habitat. The use of plant compounds to treat infection is an age. Larvicidal efficacy of medicinal plant extracts against. Extracts from leaves, flowers and roots of plants were found to have mosquito larvicidal activity sharma et al.
Larvicidal activity of some plant extracts against mosquitoes. Plant based formulations would be more feasible environmental products with proven potential as insecticide. The findings of the present investigation revealed that the leaf, bark, root and seed of carica papaya possess larvicidal and repellent. This component has potential larvicidal activity due to individual or synergistic effects on larvae.
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